《植物生理学报》 2015, 51(11): 2013-2016

风铃玉的组织培养与快速繁殖

吴正景^1,*, 黄雪娇¹, 王柏¹, 彭涛², 马文瑶¹

¹河南科技大学林学院, 河南洛阳471003; ²卢氏县中药材生产管理办公室, 河南卢氏472200

通信作者：吴正景；E-mail: wzj135@sohu.com；Tel: 0379-64282669

摘　要：

以风铃玉叶片为外植体诱导愈伤组织并分化出不定芽发生, 经生根得到再生植株, 建立风铃玉离体再生体系。风铃玉愈伤组织诱导的较好培养基为MS+NAA 0.2 mg∙L^-1+6-BA 0.2 mg∙L^-1, 诱导率为85.7%; 最佳诱芽培养基是MS+IBA 0.02 mg∙L^-1+6-BA 0.2 mg∙L^-1, 能直接诱导出生长健壮的丛生芽; 最佳生根培养基是1/2MS+IBA 0.1 mg∙L^-1, 生根率高达83.3%。

关键词：风铃玉; 叶片; 组织培养; 快速繁殖

收稿：2015-09-09   修定：2015-09-25

资助：河南科技大学博士科研基金(09001216)。

Tissue Culture and Rapid Propagation of Ophthalmophyllum friedrichiae

WU Zheng-Jing^1,*, HUANG Xue-Jiao¹, WANG Bai¹, PENG Tao², MA Wen-Yao¹

¹forestery College, Henan University of Science and Technology, Luoyang, Henan 471003, China; ²Lushi County Management Office of Herbal Medicine, Lushi, Henan 472200, China

Corresponding author: WU Zheng-Jing; E-mail: wzj135@sohu.com; Tel: 0379-64282669

Abstract:

In this paper we studied in vitro culture of Ophthalmophyllum friedrichiae by using leaf as explants, including callus induction, adventitious shoot differentiation and rooting. The results indicated that MS+NAA 0.2 mg∙L^-1+6-BA 0.2 mg∙L^-1 was the suitable medium for callus induction, with an induction rate of 85.7%. The best medium for adventitious bud differentiation was MS+IBA 0.02 mg∙L^-1+6-BA 0.2 mg∙L^-1, and the shoots induced were strong. 1/2MS+IBA 0.02 mg∙L^-1+6-BA 0.2 mg∙L^-1 was the optimum medium for rooting, and the rooting rate reached 83.3%.

Key words: Ophthalmophyllum friedrichiae; leaf; tissue culture; rapid propagation